Low matsin lamba ruwa chromatography rabuwa layering tsarin aikace-aikace kewayon: bincike da shirye-shirye na biochemistry, synthetic sunadarai, halitta kayayyakin, magunguna ci gaba, abinci sunadarai, aikin gona sunadarai, kayan ado, polymers da petrochemical da sauran masana'antu. Za a iya gudanar da ion musayar stratification, dangantaka stratification gel tacewa stratification, hydrophobic tasiri stratification da sauran hanyoyin, don rabuwa da nazarin, kwararar ganowa na furotin, enzymes, nucleic acid, nucleotide, polypeptide, (da / ba tare da aromatic amino acid) da sauran abubuwa da ke da ultraviolet sha halitta / non-halitta samfurin, shi ne kyakkyawan zaɓi na tsarkakewar kwayoyin halitta, yana da ayyuka da yawa, sauki amfani, tattalin arziki da sauran halaye. Ƙananan zane don ƙara adana sarari a cikin sanyi ajiya da dakin gwaje-gwaje.

Bayani

Abubuwan da ake buƙata don nuna alamun fasaha daban-daban na na'urar binciken UV mai haske biyu a cikin tsarin tsari ana amfani da su don gwajin ƙididdiga a masana'antun magunguna na ƙasa. Kayan aiki Features

Protein 280nm / 254nm biyu wavelength gwaji

Kwayoyin furotin suna ƙunshe da amino acids masu ƙanshi kamar tyrosine da trystane. Suna da siffofin sha ultraviolet haske, da sha kulla a 280nm wavelength, da kuma a cikin wannan wavelength sha kulla na haske yawa darajar daidai da su mayar da hankali, don haka za a iya zama a matsayin furotin inganci, adadin ma'auni tushe, saboda haka, 280nm ultraviolet sha hanyar yawanci amfani da ci gaba da saka idanu na furotin mayar da hankali a cikin layering tsarin. Amma saboda abun ciki na tyrosine da trystane na furotin daban-daban, don daidaita adadin, dole ne a kwatanta tsarki na furotin da za a gwada a matsayin ma'auni, ko kuma an riga an san ƙididdigar fadewar haske a matsayin tunani. Bugu da ƙari, yawancin abubuwan da ba na furotin ba suna da ƙarfin sha a ƙarƙashin tsawon raƙuman 280nm, wanda zai iya tsoma baki. Musamman, tasirin nucleic acid (purine da pyrimidine) ya fi tsanani. A 280nm sha ne 10 sau mafi karfi fiye da furotin (a kowace gram), amma

Nucleic acid yana da ƙarfin sha a 254nm, kuma ƙwarewar sha tana kusa da 254nm. Nucleic acid a 254nm yana da lamba sau biyu fiye da 280nm, yayin da furotin, akasin haka, 280nm UV sha ya fi 254nm sha darajar.

Yawancin

Haske sha darajar tsabta furotin: A280 / A254 "1.8

Haske sha rabo na pure nucleic acid: A280/A254 0.5

Saboda haka, lokacin da nucleic acid ke wanzu a lokaci guda a cikin mafita na furotin (mafi yawan tsarin halitta), dole ne a auna OD254nm a lokaci guda tare da OD280nm. Sa'an nan kuma daidaita ainihin abun ciki na furotin ta hanyar daidaitawar kwarewa don kawar da tasirin nucleic acid bisa ga daidaiton sha na tsawon raƙuman ruwa biyu.

Furotin mataki = 1.45 × A280-0.74 × A254 (mg / ml)

An gina wannan tsarin kwarewa ta hanyar ƙididdigar bayanai da aka ƙididdige ta hanyar haɗuwa da sunadarai daban-daban na furotin da aka sani (yeast enololase) da nucleic acid (yeast nucleic acid).

Tsarin Saituna