DWD-1 UV detector (high yi biyu haske biyu wavelengths) gina-in data sarrafawa

Wavelength kewayon: 254nm, 280nm (lokaci guda ganowa), kuma wani zaɓi biyu wavelengths ganowa lokaci guda tsakanin 254nm, 280nm-400nm layi.

* DWD-1 kayan aiki Features

Protein 280nm / 254nm biyu wavelength ƙididdiga:

Kwayoyin furotin suna ƙunshe da amino acids masu ƙanshi kamar tyrosine da trystane. Suna da siffofin sha ultraviolet haske, da sha kulla a 280nm wavelength, da kuma a cikin wannan wavelength sha kulla na haske yawa darajar daidai da su mayar da hankali, don haka za a iya zama a matsayin furotin inganci, adadin ma'auni tushe, saboda haka, 280nm ultraviolet sha hanyar yawanci amfani da ci gaba da saka idanu na furotin mayar da hankali a cikin layering tsarin. Amma saboda abun ciki na tyrosine da trystane na furotin daban-daban, don daidaita adadin, dole ne a kwatanta tsarki na furotin da za a gwada a matsayin ma'auni, ko kuma an riga an san ƙididdigar fadewar haske a matsayin tunani. Bugu da ƙari, yawancin abubuwan da ba na furotin ba suna da ƙarfin sha a ƙarƙashin tsawon raƙuman 280nm, wanda zai iya tsoma baki. Musamman, tasirin nucleic acid (purine da pyrimidine) ya fi tsanani. A 280nm, sha yana da ƙarfi sau 10 fiye da furotin (a kowace gram), amma nucleic acid yana da ƙarfi a 254nm, tare da ƙwarewar sha a kusa da 254nm. Nucleic acid dimming factor a 254nm ne sau biyu a 280nm,

Kuma furotin, akasin haka, 280nm UV sha darajar ya fi 254nm sha darajar.

Yawancin：

Haske sha darajar tsabta furotin: A280 / A254 "1.8

Hasken sha rabo na tsabtace nucleic acid: A280 / A254 ≥ 0.5

Saboda haka, lokacin da nucleic acid ke wanzu a lokaci guda a cikin mafita na furotin (mafi yawan tsarin halitta), dole ne a auna OD254nm a lokaci guda tare da OD280nm. Sa'an nan kuma daidaita ainihin abun ciki na furotin ta hanyar daidaitawar kwarewa don kawar da tasirin nucleic acid bisa ga daidaiton sha na tsawon raƙuman ruwa biyu.

Furotin mataki = 1.45 × A280-0.74 × A254 (mg / ml)

An gina wannan tsarin kwarewa ta hanyar ƙididdigar bayanai da aka ƙididdige ta hanyar haɗuwa da sunadarai daban-daban na furotin da aka sani (yeast enololase) da nucleic acid (yeast nucleic acid).