ZEISS LSM 980 tare da fasahar Airyscan 2

Sabuwar ƙarni mai inganci na tsarin hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton hoton

Don lura da ayyukan rayuwa ba tare da tsangwama ba kamar yadda zai yiwu, samfuran halittu kamar al'adun ƙwayoyin halitta masu girma uku, ƙwayoyin ƙwayoyin halitta, organoids, har ma da dukan halittu suna buƙatar alamun ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin ƙarancin Bugu da ƙari, don samun ingantaccen bayanai a ma'anar kididdiga, kuna buƙatar maimaita gwajin: Saboda haka, a nan gaba, za ku buƙaci mafi girman kwararar gwaji.

LSM 980 tare da fasahar Airyscan 2 shine kyakkyawan dandamali na hoton 4D mai mayar da hankali wanda aka inganta don gano alamun siginar rauni da ke fitar da haske har zuwa 900nm tare da ingantaccen ingancin haske, kuma yana ba da damar gano tashoshi da yawa a lokaci guda. Fasahar Airyscan 2 tare da yanayin Multiplex yana ba da ƙarin zaɓuɓɓukan hoto don gwajin ku. Kuna iya inganta saitunan don samun hotuna masu girma a cikin mafi gajeren lokacin karɓar hotuna fiye da na baya. Yawancin mataimakan software suna inganta aikin aiki don tallafawa ingantaccen tattara hoto da sarrafa bayanai.

　　Amfanin samfurin

Bayar da babban matakin imaging don bincikenku

Yi amfani da alamun fluorescence mai launi da yawa daga 380 nm zuwa 900 nm.

Yanayi mai sassauci tare da tashoshin daidaitawa har zuwa 36.

Don samun ƙarin bayani a cikin ɗan gajeren lokaci tare da Airyscan 2 Multiplex.

AI Sample Finder yana iya gano yankuna masu sha'awa da sauri.

Smart Setup yana inganta saitunan hoto da na'urar ganowa.

NLO, NIR, Cryo, da kuma hoton SIM2 za su faɗaɗa bincikenku.

　　Samun mafi kyawun bayanai da sauri

Multiplex yanayin ZEISS Airyscan 2 zai iya samun ƙarin bayani a cikin ɗan gajeren lokaci. Hasken haske da tsarin ganowa na iya karya iyakokin diffraction don cimma hoton samfurin samfurin 3D mai wuya tare da babban mitar frame, kuma ƙananan gubar haske don samfurin da ke da mahimmanci. A yau, ta hanyar haɗuwa da sassauci na mai mayar da hankali tare da bincike-a-lokaci da sauri da ƙananan gubar haske na Airyscan mai ganowa mai ƙwarewa, za ku iya samun hotuna masu ƙuduri masu ƙuduri a cikin saurin hoto sau goma don samun amsoshin tambayoyin kimiyyarka.

DNA launi (shuɗi, Hoechst 44432), microtubular launi (rawaya, fluorescent alama microtubulin antibody Alexa 488), da kuma fibroblast launi (Red, Ghost Pen Ringpeptide-Abberior STAR Red). Yanayin Multiplex yana amfani da fasahar ZEISS Airyscan 2 don yin hoto mai inganci da ƙuduri mai girma. A. Politi, J. Jakobi, da P. Lenart, Cibiyar Max Planck ta Göttingen, Jamus, sun ba da samfurin.

LSM 980 zai iya kammala daukar hoto mai wuya don samun "kifi da bear". LSM 9 jerin yana amfani da ingantattun hanyoyin haske, har zuwa tashoshi 36 tare da samfurin samfurin tare da cikakken sassauci na spectrum gami da kewayon kusan infrared (NIR). Bugu da ƙari, idan aka yi aiki tare da Airyscan 2, wannan juyin juya halin mai ganowa na fuska yana iya samun ƙarin bayani daga samfurinku a cikin ɗan gajeren lokaci. Wannan yana ba ku damar yin hoton siginar rauni tare da babban siginar amo (SNR) sau 4 - 8. Ba dole ba ne ku rage ramin allura don samun ƙuduri mai girma don yin hoton hoto mai girma.

DAPI (blue) da microtubulin antibody Alexa 700 (ja). Alexa 700 yana amfani da na'urar gano haske ta kusa da infrared. Samfurin ya bayar da Urs Ziegler da Jana Doehner daga Jami'ar Zurich ZMB, Switzerland.

　　Inganta ingancin aikin aiki

Gwajin hoto mai rikitarwa na ƙwayoyin halitta ba shi da sauƙi. ZEN software yana ba da taimako mai yawa bisa ga umarnin ku don cimma sakamakon maimaitawa a cikin gajeren lokaci.

AI Sample Finder yana taimaka maka ka gano yankunan sha'awa da sauri, yana ba ka lokaci mai yawa don yin gwaji. Smart Setup yana inganta saitunan hoto bisa ga alamar fluorescence. Aikin sarrafa bayanai kai tsaye yana ba da damar karɓar hoto da sarrafa bayanai a lokaci guda. Ko a lokacin daukar hoto ko a lokacin raba dukan gwajin a baya, ZEN Connect yana ba ku iko a kowane lokaci.

　　Airyscan fasaha ka'idodin

Na gargajiya co-focus laser bincike microscope amfani da maki haske fasahar bincike samfurin a cikin tsari. Abubuwan gani na microscope suna canza kowane maki zuwa Airy pattern. Kula rami hana non-focus jirgin sama sigina isa detector. Ƙananan ramin allura na iya samar da ƙudurin hoto mafi girma, amma farashin adadin photons da aka gano zai rage, kuma waɗannan photons ba za a iya biyan kuɗi ba ta hanyoyi kamar de-convolution.

Airyscan 2 shine mai ganowa na fuska tare da abubuwan ganowa 32 da aka tsara don taimaka muku tattara yawancin abubuwan da ke cikin Airyscan a lokaci guda. Ko'ina mai mayar da hankali na allura zai kasance a buɗe kuma ba zai hana kowane siginar shiga ba, don haka ana iya tattara ƙarin photons don samar da ƙarin haske yayin hoto. Airyscan 2 yana ba da hoto mai ƙarancin ƙuduri da ƙwarewa mai ƙarancin haske.

　　Airyscan 2 sabon Multiplex yanayin aiki

ZEISS LSM 9 jerin tare da Airyscan 2 ‍yana ba ku ƙarin zaɓuɓɓuka don biyan saurin hoto da ƙudurin hoto da ake buƙata don gwajin ku. Kuna iya haɗuwa da Co-Focus Surface Array Detector tare da haske mai hankali da nau'ikan karɓar hoto daban-daban don zaɓar daga yanayin hoto daban-daban. Sabon yanayin Multiplex yana amfani da siffofin da ke motsa spots da kuma wuraren da ke cikin rukunin mai ganowa na fuska don cire ƙarin bayanan sarari, har ma yayin karanta pixel iri ɗaya. Wannan yana ba da damar cire ƙarin bayani yayin da laser ke dubawa a kan hangen nesa, don haka inganta saurin karɓar hoto da za a iya samu. A zahiri, yawan bayanan sararin samaniya da aka kama a cikin jirgin sama na allura na iya sake gina hoton ƙarshe fiye da ƙudurin hoto na samfurin hoto. A cikin yanayin Multiplex, Airyscan 2 zai iya ɗaukar layin binciken hoto guda huɗu tare da babban SNR a lokaci guda a cikin bincike guda ɗaya. LSM 980 yana amfani da fasahar Airyscan 2 don faɗaɗa maki na laser don yin hoton layi takwas. Amfani da wannan gudun amfanin zai iya samun sauri lokaci jerin yankan single layer haske, sauri jigsaw a manyan yankuna, ko sauri lokaci jerin hoton manyan abubuwa.